Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15836 and 15837 are separated by 193 nucleotides15837 and 15838 are separated by 78 nucleotides15838 and 15839 are separated by 472 nucleotides15839 and 15840 are separated by 1040 nucleotides mRNA_7468: 15836 - Hypothetical Protein, at 351,460 to 352,308 15836 mRNA_7469: 15837 - K20403 TTI1 TELO2-interacting protein 1, at 352,502 to 356,237 15837 mRNA_7470: 15838 - K12815 DHX38, PRP16 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX38/PRP16, at 356,316 to 361,042 15838 mRNA_7471: 15839 - K00838 ARO8 aromatic amino acid aminotransferase I, at 361,515 to 364,061 15839 mRNA_7472: 15840 - K03680 EIF2B4 translation initiation factor eIF-2B subunit delta, at 365,102 to 366,661 15840
Group Condition mRNA_7468 mRNA_7469 mRNA_7470 mRNA_7471 mRNA_7472
15836 15837 15838 15839 15840
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.0 N.D.
growth on ypd YPD -0.0 N.D. N.D. -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. -0.3 N.D.
auxotrophy Dropout Complete +0.2 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. N.D. -0.2 N.D.
auxotrophy YNB Glucose +0.4 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.2 N.D.
growth on oleic acid YNB Glucose +0.5 N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.9 N.D. N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.6 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. N.D. +0.5 N.D.
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