Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15846 and 15847 are separated by 205 nucleotides15847 and 15848 are separated by 123 nucleotides15848 and 15849 are separated by 196 nucleotides15849 and 15850 are separated by 461 nucleotides mRNA_7478: 15846 - Hypothetical Protein, at 382,554 to 382,817 15846 mRNA_7479: 15847 - Hypothetical Protein, at 383,023 to 384,509 15847 mRNA_7480: 15848 - K01868 TARS, thrS threonyl-tRNA synthetase, at 384,633 to 387,547 15848 mRNA_7481: 15849 - HMMPfam-Mitochondrial domain of unknown function (DUF1713)-PF08213, at 387,744 to 389,157 15849 mRNA_7482: 15850 - Hypothetical Protein, at 389,619 to 390,464 15850
Group Condition mRNA_7478 mRNA_7479 mRNA_7480 mRNA_7481 mRNA_7482
15846 15847 15848 15849 15850
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.0 -2.5 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.0 -0.1 N.D. +0.2
auxotrophy Dropout Complete +0.1 -0.5 +0.2 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.0 -0.2 -0.0 N.D. +0.1
growth on ypd YPD +0.0 -0.3 +0.4 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.3 +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.3 +0.9 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.4 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.2 -0.2 N.D. +0.1
growth on oleic acid YNB Glucose +0.1 -0.1 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 +0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.0 +0.3 N.D. +0.6
auxotrophy YNB Glucose +0.2 -0.2 +0.3 N.D. +0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.0 +0.8 N.D. +0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.0 +0.7 N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.1 +0.6 N.D. +0.8
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 +1.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.4 +1.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.8 +0.0 +0.5 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +1.4 N.D. +0.3
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