Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15849 and 15850 are separated by 461 nucleotides15850 and 15851 are separated by 328 nucleotides15851 and 15852 are separated by 1268 nucleotides15852 and 15853 are separated by 911 nucleotides mRNA_7481: 15849 - HMMPfam-Mitochondrial domain of unknown function (DUF1713)-PF08213, at 387,744 to 389,157 15849 mRNA_7482: 15850 - Hypothetical Protein, at 389,619 to 390,464 15850 mRNA_7483: 15851 - HMMPfam-Stress-induced bacterial acidophilic repeat motif-PF10685, at 390,793 to 391,582 15851 mRNA_7484: 15852 - K09565 PPIF peptidyl-prolyl isomerase F (cyclophilin D), at 392,851 to 393,989 15852 mRNA_7485: 15853 - K08158 MDR1, FLR1, CAF5 MFS transporter, DHA1 family, multidrug resistance protein, at 394,901 to 397,101 15853
Group Condition mRNA_7481 mRNA_7482 mRNA_7483 mRNA_7484 mRNA_7485
15849 15850 15851 15852 15853
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. -0.5 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.8 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 N.D. -0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.7 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. -0.5 -0.3
growth on ypd YPD N.D. -0.2 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. +0.1 -0.4
auxotrophy Dropout Complete N.D. +0.1 N.D. +0.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.1 N.D. +0.5 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.5 N.D. -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 N.D. -0.1 -0.0
growth on oleic acid YNB Glucose N.D. +0.2 N.D. +0.5 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. +0.1 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.6 N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.2 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.7 N.D. -0.1 -0.2
auxotrophy YNB Glucose N.D. +0.5 N.D. +0.3 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.8 N.D. -0.1 +0.1
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