Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15853 and 15854 are separated by 590 nucleotides15854 and 15855 are separated by 738 nucleotides15855 and 15856 are separated by 2483 nucleotides15856 and 15857 are separated by 148 nucleotides mRNA_7485: 15853 - K08158 MDR1, FLR1, CAF5 MFS transporter, DHA1 family, multidrug resistance protein, at 394,901 to 397,101 15853 mRNA_7486: 15854 - K08287 E2.7.12.1 dual-specificity kinase, at 397,692 to 399,920 15854 mRNA_7487: 15855 - K17619 MDP1 magnesium-dependent phosphatase 1, at 400,659 to 401,356 15855 mRNA_7488: 15856 - K00558 DNMT1, dcm DNA (cytosine-5)-methyltransferase 1, at 403,840 to 407,882 15856 mRNA_7489: 15857 - K12351 SMPD2 sphingomyelin phosphodiesterase 2, at 408,031 to 409,638 15857
Group Condition mRNA_7485 mRNA_7486 mRNA_7487 mRNA_7488 mRNA_7489
15853 15854 15855 15856 15857
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -2.0 N.D. +0.4 -2.5
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.7 N.D. +0.3 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.7 N.D. -0.3 -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -1.5 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -1.1 N.D. +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.6 N.D. -0.0 -0.4
growth on oleic acid YNB Oleic Acid -0.5 -0.2 N.D. +0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -1.2 N.D. +0.2 +0.5
growth on oleic acid YNB Glucose -0.5 +0.0 N.D. +0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.6 N.D. -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 N.D. +0.2 -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.0 N.D. -0.1 -0.2
auxotrophy Dropout Complete -0.3 +0.6 N.D. -0.0 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 N.D. +0.0 +0.1
growth on ypd YPD -0.1 -0.1 N.D. +0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.6 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.4 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.8 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.3 N.D. +0.1 +0.7
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.8 N.D. +0.2 -0.3
auxotrophy YNB Glucose -0.2 +0.8 N.D. +0.2 +0.2
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