Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15855 and 15856 are separated by 2483 nucleotides15856 and 15857 are separated by 148 nucleotides15857 and 15858 are separated by 87 nucleotides15858 and 15859 are separated by 86 nucleotides mRNA_7487: 15855 - K17619 MDP1 magnesium-dependent phosphatase 1, at 400,659 to 401,356 15855 mRNA_7488: 15856 - K00558 DNMT1, dcm DNA (cytosine-5)-methyltransferase 1, at 403,840 to 407,882 15856 mRNA_7489: 15857 - K12351 SMPD2 sphingomyelin phosphodiesterase 2, at 408,031 to 409,638 15857 mRNA_7490: 15858 - K20167 TBC1D14 TBC1 domain family member 14, at 409,726 to 411,732 15858 mRNA_7491: 15859 - K19267 qorB NAD(P)H dehydrogenase (quinone), at 411,819 to 412,834 15859
Group Condition mRNA_7487 mRNA_7488 mRNA_7489 mRNA_7490 mRNA_7491
15855 15856 15857 15858 15859
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 -2.5 +0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 -1.1 -0.3 +0.0
auxotrophy Dropout Complete N.D. -0.0 -0.4 -0.5 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.3 -0.9 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 -0.9 -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 -0.4 +0.1 -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.2 -0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.1 -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.6 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.2 -0.7 +0.1
growth on oleic acid YNB Glucose N.D. +0.3 -0.4 -0.5 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.0 -0.3 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.0 -0.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.2 +0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 +0.2 -0.7 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.2 -0.6 +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.0 -0.1 -0.2 +0.1
growth on ypd YPD N.D. +0.1 +0.2 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.7 -0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 +0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +0.5 +0.0 +0.5
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