Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15856 and 15857 are separated by 148 nucleotides15857 and 15858 are separated by 87 nucleotides15858 and 15859 are separated by 86 nucleotides15859 and 15860 are separated by 1065 nucleotides mRNA_7488: 15856 - K00558 DNMT1, dcm DNA (cytosine-5)-methyltransferase 1, at 403,840 to 407,882 15856 mRNA_7489: 15857 - K12351 SMPD2 sphingomyelin phosphodiesterase 2, at 408,031 to 409,638 15857 mRNA_7490: 15858 - K20167 TBC1D14 TBC1 domain family member 14, at 409,726 to 411,732 15858 mRNA_7491: 15859 - K19267 qorB NAD(P)H dehydrogenase (quinone), at 411,819 to 412,834 15859 mRNA_7492: 15860 - KOG2301 Voltage-gated Ca2+ channels, alpha1 subunits, at 413,900 to 421,307 15860
Group Condition mRNA_7488 mRNA_7489 mRNA_7490 mRNA_7491 mRNA_7492
15856 15857 15858 15859 15860
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -2.5 +0.0 -0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -1.1 -0.3 +0.0 +0.2
auxotrophy Dropout Complete -0.0 -0.4 -0.5 -0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.3 -0.9 +0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.6 -0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.2 -0.7 +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.4 +0.1 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 -0.6 +0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.9 -0.2 +0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.2 -0.2 -0.4 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 -0.5 -0.1 -0.0
growth on oleic acid YNB Glucose +0.3 -0.4 -0.5 +0.3 -0.1
auxotrophy YNB Glucose +0.2 +0.2 -0.7 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 +0.2 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 -0.4 -0.0 +0.2
growth on ypd YPD +0.1 +0.2 -0.0 -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.0 -0.3 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.7 -0.3 -0.3 -0.1
growth on oleic acid YNB Oleic Acid +0.0 -0.1 -0.2 +0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.3 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.5 +0.0 +0.5 +0.3
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