Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15857 and 15858 are separated by 87 nucleotides15858 and 15859 are separated by 86 nucleotides15859 and 15860 are separated by 1065 nucleotides15860 and 15861 are separated by 271 nucleotides mRNA_7489: 15857 - K12351 SMPD2 sphingomyelin phosphodiesterase 2, at 408,031 to 409,638 15857 mRNA_7490: 15858 - K20167 TBC1D14 TBC1 domain family member 14, at 409,726 to 411,732 15858 mRNA_7491: 15859 - K19267 qorB NAD(P)H dehydrogenase (quinone), at 411,819 to 412,834 15859 mRNA_7492: 15860 - KOG2301 Voltage-gated Ca2+ channels, alpha1 subunits, at 413,900 to 421,307 15860 mRNA_7493: 15861 - K00489 CYP7A1 cholesterol 7alpha-monooxygenase, at 421,579 to 423,387 15861
Group Condition mRNA_7489 mRNA_7490 mRNA_7491 mRNA_7492 mRNA_7493
15857 15858 15859 15860 15861
facs on lipid accumulation Sorted Low BODIPY Gate -2.5 +0.0 -0.0 -0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 -0.3 +0.0 +0.2 -0.0
auxotrophy Dropout Complete -0.4 -0.5 -0.2 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.9 +0.0 +0.0 +0.2
growth on oleic acid YNB Glucose -0.4 -0.5 +0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.6 +0.3 -0.4 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -0.2 +0.2 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.1 -0.0 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.7 +0.1 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.1 -0.3 -0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.4 -0.1 +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.5 -0.1 -0.0 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.4 -0.0 +0.2 -0.1
auxotrophy YNB Glucose +0.2 -0.7 +0.1 -0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 -0.2 +0.1 -0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.2 +0.1 +0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.3 -0.0 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 -0.3 -0.3 -0.1 +0.0
growth on ypd YPD +0.2 -0.0 -0.1 -0.3 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 +0.1 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.0 +0.5 +0.3 -0.2
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