Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15861 and 15862 are separated by 125 nucleotides15862 and 15863 are separated by 519 nucleotides15863 and 15864 are separated by 597 nucleotides15864 and 15865 are separated by 443 nucleotides mRNA_7493: 15861 - K00489 CYP7A1 cholesterol 7alpha-monooxygenase, at 421,579 to 423,387 15861 mRNA_7494: 15862 - K05760 PXN paxillin, at 423,513 to 425,436 15862 mRNA_7495: 15863 - K10089 M6PR cation-dependent mannose-6-phosphate receptor, at 425,956 to 427,400 15863 mRNA_7496: 15864 - K03935 NDUFS2 NADH dehydrogenase (ubiquinone) Fe-S protein 2, at 427,998 to 430,216 15864 mRNA_7497: 15865 - K03685 rnc, DROSHA, RNT1 ribonuclease III, at 430,660 to 431,671 15865
Group Condition mRNA_7493 mRNA_7494 mRNA_7495 mRNA_7496 mRNA_7497
15861 15862 15863 15864 15865
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.0 -1.0 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.1 -0.6 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 -0.1 N.D. -0.5
auxotrophy Dropout Complete +0.1 -0.2 -0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.1 -0.4 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.2 -0.4 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.0 -0.4 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.5 +0.2 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.0 -0.5 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 -0.1 N.D. -0.1
growth on ypd YPD +0.5 -0.0 -0.6 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 -0.1 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.1 -0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.1 -0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.4 -0.3 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.2 +0.0 N.D. +0.2
auxotrophy YNB Glucose +0.2 -0.0 +0.2 N.D. +0.1
growth on oleic acid YNB Glucose -0.2 +0.3 +0.4 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 +0.4 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 +0.3 N.D. +0.5
growth on ricinoleic acid YNB Glucose -0.0 +0.2 +0.9 N.D. +0.5
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