Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15888 and 15889 are separated by 115 nucleotides15889 and 15890 are separated by 1223 nucleotides15890 and 15891 are separated by 366 nucleotides15891 and 15892 are separated by 246 nucleotides mRNA_7520: 15888 - K18725 POM152 nucleoporin POM152, at 482,849 to 487,263 15888 mRNA_7521: 15889 - K18624 MAEA, EMP macrophage erythroblast attacher, at 487,379 to 489,204 15889 mRNA_7522: 15890 - HMMPfam-Helix-loop-helix DNA-binding domain-PF00010,ProSiteProfiles-Myc-type, basic helix-loop-helix (bHLH) domain profile.-PS50888,SMART-helix loop helix domain-SM00353,SUPERFAMILY--SSF47459, at 490,428 to 491,714 15890 mRNA_7523: 15891 - KOG0143 Iron/ascorbate family oxidoreductases, at 492,081 to 493,296 15891 mRNA_7524: 15892 - BLAST putative heatshock protein [Serpula lacrymans var..., at 493,543 to 495,211 15892
Group Condition mRNA_7520 mRNA_7521 mRNA_7522 mRNA_7523 mRNA_7524
15888 15889 15890 15891 15892
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 -0.2 -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -1.3 +0.2 +0.1 +0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.0 +0.1 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -1.7 +0.7 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.2 -0.3 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.1 -0.5 +0.2 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 -0.1 +0.1 -0.1
auxotrophy Dropout Complete -0.1 -0.3 +0.6 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.1 +0.2 +0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 +0.4 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.0 +0.2 +0.3
auxotrophy YNB Glucose +0.0 +0.1 +0.7 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.1 +0.4 -0.0 +0.2
growth on ypd YPD +0.5 -0.3 +0.3 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.3 -0.2 +0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 +0.6 -0.1 +0.4
growth on oleic acid YNB Glucose +0.2 +0.2 +0.3 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.2 +0.3 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.0 +0.6 +0.2 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.1 +1.0 -0.0 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.1 +0.9 +0.1 +0.2
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