Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15889 and 15890 are separated by 1223 nucleotides15890 and 15891 are separated by 366 nucleotides15891 and 15892 are separated by 246 nucleotides15892 and 15893 are separated by 109 nucleotides mRNA_7521: 15889 - K18624 MAEA, EMP macrophage erythroblast attacher, at 487,379 to 489,204 15889 mRNA_7522: 15890 - HMMPfam-Helix-loop-helix DNA-binding domain-PF00010,ProSiteProfiles-Myc-type, basic helix-loop-helix (bHLH) domain profile.-PS50888,SMART-helix loop helix domain-SM00353,SUPERFAMILY--SSF47459, at 490,428 to 491,714 15890 mRNA_7523: 15891 - KOG0143 Iron/ascorbate family oxidoreductases, at 492,081 to 493,296 15891 mRNA_7524: 15892 - BLAST putative heatshock protein [Serpula lacrymans var..., at 493,543 to 495,211 15892 mRNA_7525: 15893 - KOG3425 Uncharacterized conserved protein, at 495,321 to 495,867 15893
Group Condition mRNA_7521 mRNA_7522 mRNA_7523 mRNA_7524 mRNA_7525
15889 15890 15891 15892 15893
facs on lipid accumulation Sorted No BODIPY Gate -1.3 +0.2 +0.1 +0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.2 -0.4 -0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -1.7 +0.7 +0.1 +0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 +0.1 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.4 -0.1 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.5 +0.2 +0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.3 +0.0 +0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.2 +0.0 -0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.1 -0.2 -0.3 +0.2
auxotrophy Dropout Complete -0.3 +0.6 -0.1 -0.1 -0.2
auxotrophy YNB Glucose +0.1 +0.7 -0.3 +0.1 -0.3
growth on ypd YPD -0.3 +0.3 +0.1 +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.6 +0.2 +0.6 -0.9
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 +0.1 +0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.4 -0.0 +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.2 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.6 -0.1 +0.4 -0.3
growth on oleic acid YNB Glucose +0.2 +0.3 -0.1 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 +0.2 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +1.0 -0.0 +0.5 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.9 +0.1 +0.2 +0.2
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