Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15911 and 15912 are separated by 292 nucleotides15912 and 15913 are separated by 59 nucleotides15913 and 15914 are separated by 212 nucleotides15914 and 15915 are separated by 642 nucleotides mRNA_7543: 15911 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 528,025 to 529,460 15911 mRNA_7544: 15912 - K07552 bcr MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein, at 529,753 to 531,940 15912 mRNA_7545: 15913 - Hypothetical Protein, at 532,000 to 532,304 15913 mRNA_7546: 15914 - K04523 UBQLN, DSK2 ubiquilin, at 532,517 to 534,299 15914 mRNA_7547: 15915 - Hypothetical Protein, at 534,942 to 536,762 15915
Group Condition mRNA_7543 mRNA_7544 mRNA_7545 mRNA_7546 mRNA_7547
15911 15912 15913 15914 15915
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 N.D. -0.4 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.3 N.D. -0.8 +0.0
auxotrophy YNB Glucose -0.1 -0.1 N.D. -0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.4 N.D. -0.1 -0.0
auxotrophy Dropout Complete -0.1 +0.0 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.4 N.D. -0.5 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.0 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.1 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.2 N.D. -0.3 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.5 N.D. -0.5 -0.3
growth on oleic acid YNB Glucose +0.0 +0.2 N.D. -0.0 -0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.3 N.D. -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.3 N.D. -0.4 +0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.4 N.D. -0.0 -0.2
growth on ypd YPD +0.1 -0.1 N.D. +0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.2 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.3 N.D. +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.3 N.D. +0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 N.D. +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 N.D. +0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.7 +0.2 N.D. -0.1 +0.1
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