Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15922 and 15923 are separated by 338 nucleotides15923 and 15924 are separated by 350 nucleotides15924 and 15925 are separated by 101 nucleotides15925 and 15926 are separated by 2352 nucleotides mRNA_7554: 15922 - K07359 CAMKK calcium/calmodulin-dependent protein kinase kinase, at 548,985 to 552,468 15922 mRNA_7555: 15923 - K00463 IDO, INDO indoleamine 2,3-dioxygenase, at 552,807 to 554,579 15923 mRNA_7556: 15924 - K12734 PPIL3 peptidyl-prolyl cis-trans isomerase-like 3, at 554,930 to 556,179 15924 mRNA_7557: 15925 - Hypothetical Protein, at 556,281 to 558,919 15925 mRNA_7558: 15926 - K00485 FMO dimethylaniline monooxygenase (N-oxide forming), at 561,272 to 563,698 15926
Group Condition mRNA_7554 mRNA_7555 mRNA_7556 mRNA_7557 mRNA_7558
15922 15923 15924 15925 15926
facs on lipid accumulation Sorted Low BODIPY Gate -2.0 -0.0 -1.1 -1.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.5 -0.1 -0.6 -0.6 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -2.2 +0.0 +0.0 -0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.2 -0.4 +0.0 +0.7 -0.5
growth on ricinoleic acid YNB Glucose -2.6 -0.1 +0.2 +0.4 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 +0.1 -1.2 +0.2 +0.2
growth on oleic acid YNB Oleic Acid -0.6 -0.5 -0.5 -0.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.5 -0.2 +0.1 -0.2 -0.2
growth on oleic acid YNB Glucose -1.1 -0.3 -0.3 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.8 -0.3 -0.2 +0.5 +0.1
auxotrophy YNB Glucose -0.8 -0.1 -0.3 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.2 -0.1 -0.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 -0.6 +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.7 +0.3 -0.4 -1.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 +0.2 -0.5 -0.3
auxotrophy Dropout Complete +0.6 -0.4 +0.2 -0.3 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.6 +0.2 -0.5 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.1 -0.3 +0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine +1.3 -0.5 -0.5 -0.2 -0.2
growth on ypd YPD -0.8 -0.1 +0.4 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 -0.1 +0.4 +0.1
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