Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15941 and 15942 are separated by 246 nucleotides15942 and 15943 are separated by 572 nucleotides15943 and 15944 are separated by 45 nucleotides15944 and 15945 are separated by 85 nucleotides mRNA_7573: 15941 - KOG0331 ATP-dependent RNA helicase, at 597,101 to 600,100 15941 mRNA_7574: 15942 - K15275 SLC35B1 solute carrier family 35 (UDP-galactose transporter), member B1, at 600,347 to 601,742 15942 mRNA_7575: 15943 - HMMPfam-Tetratricopeptide repeat-PF00515,HMMPfam-Tetratricopeptide repeat-PF13371,ProSiteProfiles-TPR repeat region circular profile.-PS50293,SMART-Tetratricopeptide repeats-SM00028,SUPERFAMILY--SSF48452, at 602,315 to 604,765 15943 mRNA_7576: 15944 - K02838 frr, MRRF, RRF ribosome recycling factor, at 604,811 to 605,906 15944 mRNA_7577: 15945 - Hypothetical Protein, at 605,992 to 606,645 15945
Group Condition mRNA_7573 mRNA_7574 mRNA_7575 mRNA_7576 mRNA_7577
15941 15942 15943 15944 15945
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -0.0 N.D. -2.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.3 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.3 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.2 N.D. +0.2
auxotrophy Dropout Complete +0.3 N.D. -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.1 N.D. +0.4
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.3 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +0.2 N.D. +0.1
growth on oleic acid YNB Glucose -0.0 N.D. +0.1 N.D. +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.5 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.0 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.1 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. +0.2 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.0 N.D. +0.5
growth on ypd YPD +0.3 N.D. +0.3 N.D. +0.1
auxotrophy YNB Glucose +0.1 N.D. +0.1 N.D. +0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.3 N.D. +0.7
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