Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15948 and 15949 are separated by 410 nucleotides15949 and 15950 are separated by 855 nucleotides15950 and 15951 are separated by 61 nucleotides15951 and 15952 are separated by 294 nucleotides mRNA_7580: 15948 - KOG1471 Phosphatidylinositol transfer protein SEC14 and related proteins, at 609,831 to 611,279 15948 mRNA_7581: 15949 - HMMPfam-Alpha/beta hydrolase family-PF12697,SUPERFAMILY--SSF53474, at 611,690 to 614,027 15949 mRNA_7582: 15950 - KOG2534 DNA polymerase IV (family X), at 614,883 to 616,136 15950 mRNA_7583: 15951 - HMMPfam-Polysaccharide deacetylase-PF01522,ProSiteProfiles-NodB homology domain profile.-PS51677,SUPERFAMILY--SSF88713, at 616,198 to 617,544 15951 mRNA_7584: 15952 - KOG0546 HSP90 co-chaperone CPR7/Cyclophilin, at 617,839 to 618,826 15952
Group Condition mRNA_7580 mRNA_7581 mRNA_7582 mRNA_7583 mRNA_7584
15948 15949 15950 15951 15952
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.3 -0.1 -1.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.1 -0.1 -0.8
growth on ypd YPD N.D. +0.5 -0.5 -0.4 -0.2
auxotrophy YNB Glucose N.D. +0.1 +0.3 -0.0 -1.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 -0.2 +0.0 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 -0.4 +0.4 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 +0.1 +0.3 -0.7
auxotrophy Dropout Complete N.D. -0.0 +0.2 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 -0.2 +0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 -0.2 -0.4 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.0 -0.3 +0.7
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 +0.5 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 +0.1 +0.9 -0.0
growth on oleic acid YNB Glucose N.D. +0.3 +0.3 +0.4 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.4 +0.6 -0.5
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.0 +0.9 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.0 +1.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.2 +0.6 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 -0.1 +1.3 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.1 +0.8 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +0.4 +0.8 +0.2
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