Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15976 and 15977 are separated by 65 nucleotides15977 and 15978 are separated by 353 nucleotides15978 and 15979 are separated by 361 nucleotides15979 and 15980 are separated by 550 nucleotides mRNA_7608: 15976 - K10847 XPA DNA-repair protein complementing XP-A cells, at 666,565 to 667,853 15976 mRNA_7609: 15977 - KOG3239 Density-regulated protein related to translation initiation factor 1 (eIF-1/SUI1), at 667,919 to 668,964 15977 mRNA_7610: 15978 - KOG0548 Molecular co-chaperone STI1, at 669,318 to 671,371 15978 mRNA_7611: 15979 - KOG4779 Predicted membrane protein, at 671,733 to 672,150 15979 mRNA_7612: 15980 - Hypothetical Protein, at 672,701 to 674,569 15980
Group Condition mRNA_7608 mRNA_7609 mRNA_7610 mRNA_7611 mRNA_7612
15976 15977 15978 15979 15980
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. -0.4 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -1.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.6 N.D. -0.6
growth on oleic acid YNB Glucose -0.5 N.D. -0.6 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. -0.7 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.5 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.1 N.D. -0.4
auxotrophy Dropout Complete +0.2 N.D. -0.4 N.D. -0.0
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.1 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.4 N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.1 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.3 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. +0.3 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.2 N.D. +0.5
auxotrophy YNB Glucose +0.1 N.D. -0.0 N.D. +0.6
growth on ypd YPD +0.6 N.D. -0.3 N.D. +0.6
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +0.3 N.D. +0.4
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