Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15996 and 15997 are separated by 129 nucleotides15997 and 15998 are separated by 105 nucleotides15998 and 15999 are separated by 121 nucleotides15999 and 16000 are separated by 801 nucleotides mRNA_7628: 15996 - KOG1721 FOG Zn-finger, at 712,863 to 715,455 15996 mRNA_7629: 15997 - K20474 RINT1, TIP20 RAD50-interacting protein 1, at 715,585 to 720,215 15997 mRNA_7630: 15998 - K11353 NDUFA13 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 13, at 720,321 to 721,070 15998 mRNA_7631: 15999 - KOG0226 RNA-binding proteins, at 721,192 to 722,141 15999 mRNA_7632: 16000 - KOG0383 Predicted helicase, at 722,943 to 730,457 16000
Group Condition mRNA_7628 mRNA_7629 mRNA_7630 mRNA_7631 mRNA_7632
15996 15997 15998 15999 16000
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.3 N.D. -3.0 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.1 N.D. -1.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.0 N.D. -1.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.2 N.D. -0.4 -1.0
ypd buoyancy 40 hrs Low Buoyancy -0.6 -0.1 N.D. -1.0 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 N.D. -1.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.0 N.D. -1.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.4 N.D. -0.6 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.3 N.D. -0.1 +0.1
growth on ypd YPD -0.6 +0.0 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.3 N.D. -0.1 -0.1
auxotrophy YNB Glucose +0.0 -0.3 N.D. -0.3 +0.1
growth on oleic acid YNB Oleic Acid -0.2 +0.0 N.D. +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Glucose +0.2 +0.2 N.D. -0.7 -0.0
auxotrophy Dropout Complete -0.1 -0.3 N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.3 N.D. +0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.0 N.D. +0.1 -0.5
growth on oleic acid YNB Glucose -0.1 +0.2 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.1 N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.0 N.D. +0.1 +0.2
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