Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16012 and 16013 are separated by 697 nucleotides16013 and 16014 are separated by 38 nucleotides16014 and 16015 are separated by 66 nucleotides16015 and 16016 are separated by 647 nucleotides mRNA_7644: 16012 - KOG0118 FOG RRM domain, at 754,118 to 756,054 16012 mRNA_7645: 16013 - K04794 PTH2 peptidyl-tRNA hydrolase, PTH2 family, at 756,752 to 757,515 16013 mRNA_7646: 16014 - Hypothetical Protein, at 757,554 to 759,024 16014 mRNA_7647: 16015 - KOG2741 Dimeric dihydrodiol dehydrogenase, at 759,091 to 760,573 16015 mRNA_7648: 16016 - K01705 LYS4 homoaconitate hydratase, at 761,221 to 763,593 16016
Group Condition mRNA_7644 mRNA_7645 mRNA_7646 mRNA_7647 mRNA_7648
16012 16013 16014 16015 16016
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.0 -0.0 -4.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 -0.2 -3.7
growth on oleic acid YNB Glucose N.D. N.D. +0.3 +0.0 -4.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 +0.1 -3.5
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 +0.3 -3.8
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 -0.2 -3.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.6 +0.7 -3.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.3 -3.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.8 -1.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.0 -1.1 -1.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.2 +0.3 -3.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.3 -0.4 -1.7
auxotrophy YNB Glucose N.D. N.D. +0.2 +0.9 -3.5
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 +0.5 -3.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 -0.4 -1.5
auxotrophy Dropout Complete N.D. N.D. +0.2 +0.4 -0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.4 -0.3
growth on ypd YPD N.D. N.D. -0.3 +0.5 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 +0.3 +0.8
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.6 +1.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +1.3 +0.5
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