Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16016 and 16017 are separated by 412 nucleotides16017 and 16018 are separated by 169 nucleotides16018 and 16019 are separated by 725 nucleotides16019 and 16020 are separated by 316 nucleotides mRNA_7648: 16016 - K01705 LYS4 homoaconitate hydratase, at 761,221 to 763,593 16016 mRNA_7649: 16017 - K13346 PEX10 peroxin-10, at 764,006 to 765,401 16017 mRNA_7650: 16018 - K08008 NOX, GP91 NADPH oxidase, at 765,571 to 768,508 16018 mRNA_7651: 16019 - K03671 trxA thioredoxin 1, at 769,234 to 769,814 16019 mRNA_7652: 16020 - HMMPfam-XPG I-region-PF00867,PRINTS-Xeroderma pigmentosum group G/yeast RAD superfamily signature-PR00853,SMART-Xeroderma pigmentosum G I-region-SM00484,SUPERFAMILY--SSF47807,SUPERFAMILY--SSF88723, at 770,131 to 772,818 16020
Group Condition mRNA_7648 mRNA_7649 mRNA_7650 mRNA_7651 mRNA_7652
16016 16017 16018 16019 16020
growth on ricinoleic acid YNB Ricinoleic Acid -4.0 N.D. +0.1 -0.3 -0.1
growth on oleic acid YNB Glucose -4.1 N.D. +0.3 -0.5 -0.0
growth on ricinoleic acid YNB Glucose -3.8 N.D. +0.1 -0.4 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -3.5 N.D. +0.0 -0.7 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -3.7 N.D. +0.2 -0.4 -0.2
auxotrophy YNB Glucose -3.5 N.D. +0.1 -0.5 -0.1
auxotrophy YNB Glucose 75mM Arginine -3.4 N.D. +0.2 -0.6 -0.1
auxotrophy YNB Glucose 75mM Methionine -3.0 N.D. +0.1 -0.7 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -3.5 N.D. +0.1 -0.7 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -3.0 N.D. +0.1 -0.6 +0.1
growth on oleic acid YNB Oleic Acid -3.4 N.D. +0.3 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.7 N.D. -0.0 -0.7 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.5 N.D. -0.0 -0.6 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.5 N.D. -0.1 -0.6 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.6 N.D. +0.0 -0.3 +0.5
auxotrophy Dropout Complete -0.7 N.D. +0.1 -0.4 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -0.3 -0.7 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.2 +0.2 -0.1
growth on ypd YPD +0.1 N.D. +0.0 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +1.1 N.D. +0.0 +0.5 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.8 N.D. +0.0 +0.9 -0.0
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