Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16019 and 16020 are separated by 316 nucleotides16020 and 16021 are separated by 276 nucleotides16021 and 16022 are separated by 566 nucleotides16022 and 16023 are separated by 389 nucleotides mRNA_7651: 16019 - K03671 trxA thioredoxin 1, at 769,234 to 769,814 16019 mRNA_7652: 16020 - HMMPfam-XPG I-region-PF00867,PRINTS-Xeroderma pigmentosum group G/yeast RAD superfamily signature-PR00853,SMART-Xeroderma pigmentosum G I-region-SM00484,SUPERFAMILY--SSF47807,SUPERFAMILY--SSF88723, at 770,131 to 772,818 16020 mRNA_7653: 16021 - K15216 RRN3, TIFIA RNA polymerase I-specific transcription initiation factor RRN3, at 773,095 to 776,030 16021 mRNA_7654: 16022 - K06911 K06911 uncharacterized protein, at 776,597 to 777,755 16022 mRNA_7655: 16023 - K12194 CHMP4, SNF7, VPS32 charged multivesicular body protein 4, at 778,145 to 779,164 16023
Group Condition mRNA_7651 mRNA_7652 mRNA_7653 mRNA_7654 mRNA_7655
16019 16020 16021 16022 16023
facs on lipid accumulation Sorted No BODIPY Gate -0.7 +0.1 N.D. -1.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.1 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.7 +0.5 N.D. -0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.7 -0.3 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.4 -0.1 N.D. -0.1 N.D.
auxotrophy YNB Glucose -0.5 -0.1 N.D. -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -0.1 N.D. +0.2 N.D.
auxotrophy Dropout Complete -0.4 -0.3 N.D. +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.1 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 +0.2 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.6 -0.1 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.2 N.D. +0.3 N.D.
growth on oleic acid YNB Glucose -0.5 -0.0 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.5 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 +0.5 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.1 N.D. +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.0 -0.2 N.D. +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 N.D. +0.0 N.D.
growth on ypd YPD +0.1 -0.2 N.D. +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.0 N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.9 -0.0 N.D. +0.2 N.D.
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