Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16020 and 16021 are separated by 276 nucleotides16021 and 16022 are separated by 566 nucleotides16022 and 16023 are separated by 389 nucleotides16023 and 16024 are separated by 458 nucleotides mRNA_7652: 16020 - HMMPfam-XPG I-region-PF00867,PRINTS-Xeroderma pigmentosum group G/yeast RAD superfamily signature-PR00853,SMART-Xeroderma pigmentosum G I-region-SM00484,SUPERFAMILY--SSF47807,SUPERFAMILY--SSF88723, at 770,131 to 772,818 16020 mRNA_7653: 16021 - K15216 RRN3, TIFIA RNA polymerase I-specific transcription initiation factor RRN3, at 773,095 to 776,030 16021 mRNA_7654: 16022 - K06911 K06911 uncharacterized protein, at 776,597 to 777,755 16022 mRNA_7655: 16023 - K12194 CHMP4, SNF7, VPS32 charged multivesicular body protein 4, at 778,145 to 779,164 16023 mRNA_7656: 16024 - Hypothetical Protein, at 779,623 to 780,980 16024
Group Condition mRNA_7652 mRNA_7653 mRNA_7654 mRNA_7655 mRNA_7656
16020 16021 16022 16023 16024
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -1.0 N.D. -0.1
auxotrophy Dropout Complete -0.3 N.D. +0.1 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.6 N.D. -0.4
auxotrophy YNB Glucose -0.1 N.D. -0.0 N.D. -0.3
growth on ypd YPD -0.2 N.D. +0.4 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.0 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.2 N.D. +0.3 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.2 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.1 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.2 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.0 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.2 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.2 N.D. +0.5
growth on oleic acid YNB Glucose -0.0 N.D. +0.3 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.3 N.D. +1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.0 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.4 N.D. +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.3 N.D. +1.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.5 N.D. +1.1
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