Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16050 and 16051 are separated by 265 nucleotides16051 and 16052 are separated by 191 nucleotides16052 and 16053 are separated by 742 nucleotides16053 and 16054 are separated by 817 nucleotides mRNA_7682: 16050 - Hypothetical Protein, at 827,950 to 829,213 16050 mRNA_7683: 16051 - K02542 MCM6 DNA replication licensing factor MCM6, at 829,479 to 832,868 16051 mRNA_7684: 16052 - Hypothetical Protein, at 833,060 to 834,277 16052 mRNA_7685: 16053 - Hypothetical Protein, at 835,020 to 835,589 16053 mRNA_7686: 16054 - K06185 ABCF2 ATP-binding cassette, subfamily F, member 2, at 836,407 to 838,350 16054
Group Condition mRNA_7682 mRNA_7683 mRNA_7684 mRNA_7685 mRNA_7686
16050 16051 16052 16053 16054
facs on lipid accumulation Sorted No BODIPY Gate -0.5 N.D. -0.7 -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. -0.3 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.3 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. -0.1 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.2 +0.2 N.D.
growth on ypd YPD -0.2 N.D. -0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.1 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.3 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.0 +0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. +0.3 +0.1 N.D.
auxotrophy YNB Glucose +0.5 N.D. -0.2 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.4 +0.1 N.D.
auxotrophy Dropout Complete +0.6 N.D. -0.0 +0.1 N.D.
growth on oleic acid YNB Glucose +0.3 N.D. +0.3 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. +0.1 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. +0.2 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.2 +0.5 N.D.
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