Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16053 and 16054 are separated by 817 nucleotides16054 and 16055 are separated by 153 nucleotides16055 and 16056 are separated by 103 nucleotides16056 and 16057 are separated by 209 nucleotides mRNA_7685: 16053 - Hypothetical Protein, at 835,020 to 835,589 16053 mRNA_7686: 16054 - K06185 ABCF2 ATP-binding cassette, subfamily F, member 2, at 836,407 to 838,350 16054 mRNA_7687: 16055 - SUPERFAMILY--SSF48371, at 838,504 to 840,672 16055 mRNA_7688: 16056 - Hypothetical Protein, at 840,776 to 844,586 16056 mRNA_7689: 16057 - BLAST triacylglycerol lipase [Pochonia chlamydosporia 170], at 844,796 to 845,727 16057
Group Condition mRNA_7685 mRNA_7686 mRNA_7687 mRNA_7688 mRNA_7689
16053 16054 16055 16056 16057
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.2 -0.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.3 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.2 -0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. -0.2 +0.0 +0.2
growth on ypd YPD +0.3 N.D. +0.1 -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.0 +0.1 -0.2
auxotrophy Dropout Complete +0.1 N.D. +0.1 -0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. -0.1 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.1 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. -0.0 +0.0 +0.2
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.0 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.1 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.0 -0.2 +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.0 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.2 -0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. -0.0 +0.3 +0.5
growth on oleic acid YNB Glucose +0.2 N.D. -0.0 -0.1 +0.5
auxotrophy YNB Glucose +0.1 N.D. +0.2 -0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.1 +0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 N.D. -0.2 +0.0 +0.8
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