Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16054 and 16055 are separated by 153 nucleotides16055 and 16056 are separated by 103 nucleotides16056 and 16057 are separated by 209 nucleotides16057 and 16058 are separated by 519 nucleotides mRNA_7686: 16054 - K06185 ABCF2 ATP-binding cassette, subfamily F, member 2, at 836,407 to 838,350 16054 mRNA_7687: 16055 - SUPERFAMILY--SSF48371, at 838,504 to 840,672 16055 mRNA_7688: 16056 - Hypothetical Protein, at 840,776 to 844,586 16056 mRNA_7689: 16057 - BLAST triacylglycerol lipase [Pochonia chlamydosporia 170], at 844,796 to 845,727 16057 mRNA_7690: 16058 - BLAST proteophosphoglycan ppg4 [Moesziomyces aphidis DSM 70..., at 846,247 to 848,747 16058
Group Condition mRNA_7686 mRNA_7687 mRNA_7688 mRNA_7689 mRNA_7690
16054 16055 16056 16057 16058
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.2 -0.2 -0.1
growth on ypd YPD N.D. +0.1 -0.2 -0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 -0.1 +0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 +0.1 -0.1 -0.3
auxotrophy Dropout Complete N.D. +0.1 -0.3 +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.2 +0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.2 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.0 +0.2 -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.0 -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.1 +0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.0 +0.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.0 +0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.1 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.0 +0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 +0.1 -0.2 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.1 +0.3 +0.0
growth on oleic acid YNB Glucose N.D. -0.0 -0.1 +0.5 -0.1
auxotrophy YNB Glucose N.D. +0.2 -0.2 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.3 +0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 +0.0 +0.8 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.3 +0.5 +0.0
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