Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16088 and 16089 are separated by 112 nucleotides16089 and 16090 are separated by 240 nucleotides16090 and 16091 are separated by 393 nucleotides16091 and 16092 are separated by 246 nucleotides mRNA_7720: 16088 - K03177 truB, PUS4, TRUB1 tRNA pseudouridine55 synthase, at 923,963 to 925,405 16088 mRNA_7721: 16089 - K19998 SCFD1, SLY1 sec1 family domain-containing protein 1, at 925,518 to 928,664 16089 mRNA_7722: 16090 - BLAST iQ calmodulin-binding motif protein [Moesziomyces..., at 928,905 to 930,722 16090 mRNA_7723: 16091 - HMMPfam-Domain of unknown function (DUF427)-PF04248, at 931,116 to 931,814 16091 mRNA_7724: 16092 - KOG2644 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes, at 932,061 to 933,452 16092
Group Condition mRNA_7720 mRNA_7721 mRNA_7722 mRNA_7723 mRNA_7724
16088 16089 16090 16091 16092
growth on ypd YPD -0.5 N.D. +0.1 -0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.2 -0.6 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.1 -0.5 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.1 +0.3 -0.1
auxotrophy Dropout Complete -0.0 N.D. +0.0 -0.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.1 +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.4 -0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.0 -0.4 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.3 -0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.2 -0.6 +0.3
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 +0.1 -0.2
auxotrophy YNB Glucose +0.0 N.D. +0.0 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.1 +0.1 +0.3
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.1 +0.5 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.2 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.3 +0.4 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 +0.1 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.1 +0.8 +0.5
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.1 +0.4 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.1 +0.8 +0.1
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