Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16103 and 16104 are separated by 1353 nucleotides16104 and 16105 are separated by 583 nucleotides16105 and 16106 are separated by 148 nucleotides16106 and 16107 are separated by 276 nucleotides mRNA_7735: 16103 - Hypothetical Protein, at 954,203 to 954,907 16103 mRNA_7736: 16104 - HMMPfam-Glyoxal oxidase N-terminus-PF07250,HMMPfam-Domain of unknown function (DUF1929)-PF09118,SUPERFAMILY--SSF50965,SUPERFAMILY--SSF81296, at 956,261 to 958,930 16104 mRNA_7737: 16105 - HMMPfam-Antibiotic biosynthesis monooxygenase-PF03992,SUPERFAMILY--SSF54909, at 959,514 to 960,315 16105 mRNA_7738: 16106 - HMMPfam-BTB/POZ domain-PF00651,ProSiteProfiles-BTB domain profile.-PS50097, at 960,464 to 961,733 16106 mRNA_7739: 16107 - K20476 RIC1 RAB6A-GEF complex partner protein 1, at 962,010 to 966,625 16107
Group Condition mRNA_7735 mRNA_7736 mRNA_7737 mRNA_7738 mRNA_7739
16103 16104 16105 16106 16107
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.0 +0.5 -2.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.3 +0.7 -2.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -1.9 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -1.0 N.D. -0.2 +0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. -0.3 +0.1 -0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.6 +0.1 -1.7
auxotrophy YNB Glucose -0.5 N.D. -0.3 +0.4 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.5 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.6 -0.1 -1.1
growth on ypd YPD +0.0 N.D. -0.9 +0.7 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +1.1 -0.2 -1.6
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.5 -0.5 -0.1
auxotrophy Dropout Complete -0.1 N.D. +0.3 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.6 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +1.2 +0.6 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.9 +0.4 -0.6
growth on oleic acid YNB Glucose +0.5 N.D. +0.5 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. +1.1 -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.7 +0.6 -0.0
growth on ricinoleic acid YNB Glucose -0.4 N.D. +1.1 +0.8 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.7 +0.4 +0.5
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