Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16109 and 16110 are separated by 239 nucleotides16110 and 16111 are separated by 441 nucleotides16111 and 16112 are separated by 285 nucleotides16112 and 16113 are separated by 1398 nucleotides mRNA_7741: 16109 - Hypothetical Protein, at 968,367 to 969,216 16109 mRNA_7742: 16110 - KOG3630 Nuclear pore complex, Nup214/CAN component, at 969,456 to 971,369 16110 mRNA_7743: 16111 - K09569 FKBP2 FK506-binding protein 2, at 971,811 to 972,530 16111 mRNA_7744: 16112 - Hypothetical Protein, at 972,816 to 973,446 16112 mRNA_7745: 16113 - Hypothetical Protein, at 974,845 to 975,369 16113
Group Condition mRNA_7741 mRNA_7742 mRNA_7743 mRNA_7744 mRNA_7745
16109 16110 16111 16112 16113
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -1.0 -1.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.1 -1.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. -0.4 -0.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.4 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.4 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.3 +0.5 N.D.
auxotrophy Dropout Complete +0.7 N.D. -0.5 -0.4 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.3 +0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.2 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.8 N.D. +0.7 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. +0.4 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 N.D. +0.5 +0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.0 +0.2 N.D.
auxotrophy YNB Glucose +1.1 N.D. -0.2 -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.5 +0.2 N.D.
growth on oleic acid YNB Oleic Acid +0.6 N.D. +0.2 +0.2 N.D.
growth on oleic acid YNB Glucose +0.6 N.D. +0.2 +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. -0.1 +0.8 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.2 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. +0.3 +1.6 N.D.
growth on ypd YPD +1.0 N.D. +0.5 +0.3 N.D.
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