Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16128 and 16129 are separated by 186 nucleotides16129 and 16130 are separated by 129 nucleotides16130 and 16131 are separated by 225 nucleotides16131 and 16132 are separated by 870 nucleotides mRNA_7760: 16128 - K05607 AUH methylglutaconyl-CoA hydratase, at 1,005,375 to 1,006,761 16128 mRNA_7761: 16129 - KOG1176 Acyl-CoA synthetase, at 1,006,948 to 1,009,515 16129 mRNA_7762: 16130 - K20302 TRAPPC3, BET3 trafficking protein particle complex subunit 3, at 1,009,645 to 1,010,524 16130 mRNA_7763: 16131 - HMMPfam-Bestrophin, RFP-TM, chloride channel-PF01062, at 1,010,750 to 1,013,184 16131 mRNA_7764: 16132 - Hypothetical Protein, at 1,014,055 to 1,015,066 16132
Group Condition mRNA_7760 mRNA_7761 mRNA_7762 mRNA_7763 mRNA_7764
16128 16129 16130 16131 16132
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 +0.2 N.D. -0.4 -1.2
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.0 N.D. +0.2 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 -0.4 N.D. -0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.0 N.D. -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.0 N.D. -0.0 -0.7
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.3 N.D. +0.2 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.4 N.D. +0.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.2 N.D. -0.2 +0.4
growth on ypd YPD +0.2 -0.2 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.3 N.D. -0.0 +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 N.D. +0.2 +0.3
auxotrophy YNB Glucose -0.0 +0.4 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.3 N.D. +0.4 +0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.1 N.D. +0.2 +0.5
growth on ricinoleic acid YNB Glucose +0.3 +0.0 N.D. +0.2 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.1 N.D. +0.2 +0.1
growth on oleic acid YNB Glucose +0.2 +0.2 N.D. +0.1 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.2 N.D. +0.2 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.4 N.D. +0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.3 N.D. +0.2 +1.3
auxotrophy Dropout Complete +0.3 +0.4 N.D. +0.2 +0.6
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