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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
16128 and 16129 are separated by 186 nucleotides
16129 and 16130 are separated by 129 nucleotides
16130 and 16131 are separated by 225 nucleotides
16131 and 16132 are separated by 870 nucleotides
mRNA_7760: 16128 - K05607 AUH methylglutaconyl-CoA hydratase, at 1,005,375 to 1,006,761
16128
mRNA_7761: 16129 - KOG1176 Acyl-CoA synthetase, at 1,006,948 to 1,009,515
16129
mRNA_7762: 16130 - K20302 TRAPPC3, BET3 trafficking protein particle complex subunit 3, at 1,009,645 to 1,010,524
16130
mRNA_7763: 16131 - HMMPfam-Bestrophin, RFP-TM, chloride channel-PF01062, at 1,010,750 to 1,013,184
16131
mRNA_7764: 16132 - Hypothetical Protein, at 1,014,055 to 1,015,066
16132
Group
Condition
mRNA
_7760
mRNA
_7761
mRNA
_7762
mRNA
_7763
mRNA
_7764
16128
16129
16130
16131
16132
ypd buoyancy
40 hrs No Buoyancy Separation
-1.0
+0.2
N.D.
-0.4
-1.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.5
-0.0
N.D.
+0.2
-0.5
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.6
-0.4
N.D.
-0.1
+0.3
ypd buoyancy
40 hrs High Buoyancy
-0.3
-0.0
N.D.
-0.1
-0.2
ypd buoyancy
40 hrs Low Buoyancy
+0.4
+0.0
N.D.
-0.0
-0.7
auxotrophy
YNB Glucose 75mM Methionine
+0.0
+0.3
N.D.
+0.2
-0.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.3
-0.4
N.D.
+0.1
+0.5
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
-0.2
N.D.
-0.2
+0.4
growth on ypd
YPD
+0.2
-0.2
N.D.
+0.2
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.3
N.D.
-0.0
+0.4
ypd buoyancy
40 hrs Median Buoyancy
+0.1
-0.1
N.D.
+0.2
+0.3
auxotrophy
YNB Glucose
-0.0
+0.4
N.D.
+0.1
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
+0.3
N.D.
+0.4
+0.3
growth on oleic acid
YNB Oleic Acid
-0.0
+0.1
N.D.
+0.2
+0.5
growth on ricinoleic acid
YNB Glucose
+0.3
+0.0
N.D.
+0.2
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.5
+0.1
N.D.
+0.2
+0.1
growth on oleic acid
YNB Glucose
+0.2
+0.2
N.D.
+0.1
+0.5
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.2
+0.2
N.D.
+0.2
+0.5
auxotrophy
YNB Glucose 75mM Arginine
+0.1
+0.4
N.D.
+0.2
+0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
+0.3
N.D.
+0.2
+1.3
auxotrophy
Dropout Complete
+0.3
+0.4
N.D.
+0.2
+0.6
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