Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16141 and 16142 are separated by 194 nucleotides16142 and 16143 are separated by 863 nucleotides16143 and 16144 are separated by 221 nucleotides16144 and 16145 are separated by 392 nucleotides mRNA_7773: 16141 - K12857 SNRNP40, PRP8BP Prp8 binding protein, at 1,031,798 to 1,033,403 16141 mRNA_7774: 16142 - KOG1546 Metacaspase involved in regulation of apoptosis, at 1,033,598 to 1,035,909 16142 mRNA_7775: 16143 - K01673 cynT, can carbonic anhydrase, at 1,036,773 to 1,037,366 16143 mRNA_7776: 16144 - K01899 LSC1 succinyl-CoA synthetase alpha subunit, at 1,037,588 to 1,039,295 16144 mRNA_7777: 16145 - KOG0769 Predicted mitochondrial carrier protein, at 1,039,688 to 1,041,157 16145
Group Condition mRNA_7773 mRNA_7774 mRNA_7775 mRNA_7776 mRNA_7777
16141 16142 16143 16144 16145
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.3 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.2 +0.0 +0.2 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 +0.2 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.3 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.4 -0.0 +0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.7 +0.1 +0.3 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.3 +0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.1 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.3 +0.2 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 +0.3 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 +0.2 N.D. N.D.
growth on ypd YPD +0.5 +0.1 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 +0.3 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.0 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.5 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.4 +0.3 N.D. N.D.
auxotrophy YNB Glucose +0.3 +0.4 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 -0.1 +0.1 N.D. N.D.
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