Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16145 and 16146 are separated by 31 nucleotides16146 and 16147 are separated by 747 nucleotides16147 and 16148 are separated by 687 nucleotides16148 and 16149 are separated by 36 nucleotides mRNA_7777: 16145 - KOG0769 Predicted mitochondrial carrier protein, at 1,039,688 to 1,041,157 16145 mRNA_7778: 16146 - Hypothetical Protein, at 1,041,189 to 1,043,169 16146 mRNA_7779: 16147 - K06655 PHO85 negative regulator of the PHO system, at 1,043,917 to 1,045,287 16147 mRNA_7780: 16148 - KOG0376 Serine-threonine phosphatase 2A, catalytic subunit, at 1,045,975 to 1,050,892 16148 mRNA_7781: 16149 - K18664 SLH1 antiviral helicase SLH1, at 1,050,929 to 1,056,955 16149
Group Condition mRNA_7777 mRNA_7778 mRNA_7779 mRNA_7780 mRNA_7781
16145 16146 16147 16148 16149
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 N.D. +0.1 -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. +0.1 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 N.D. +0.2 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 N.D. -0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 N.D. +0.1 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 N.D. +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.2 -0.4
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.1 -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.2 -0.4
auxotrophy Dropout Complete N.D. -0.2 N.D. +0.2 -0.1
growth on ypd YPD N.D. -0.0 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. +0.0 -0.1
growth on oleic acid YNB Glucose N.D. -0.1 N.D. +0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.3 -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 N.D. +0.3 -0.2
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