Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16180 and 16181 are separated by 401 nucleotides16181 and 16182 are separated by 867 nucleotides16182 and 16183 are separated by 430 nucleotides16183 and 16184 are separated by 321 nucleotides mRNA_7812: 16180 - K12876 RBM8A, Y14 RNA-binding protein 8A, at 1,127,228 to 1,128,549 16180 mRNA_7813: 16181 - K01476 E3.5.3.1, rocF, arg arginase, at 1,128,951 to 1,130,543 16181 mRNA_7814: 16182 - K01620 ltaE threonine aldolase, at 1,131,411 to 1,132,941 16182 mRNA_7815: 16183 - Hypothetical Protein, at 1,133,372 to 1,134,436 16183 mRNA_7816: 16184 - Hypothetical Protein, at 1,134,758 to 1,135,633 16184
Group Condition mRNA_7812 mRNA_7813 mRNA_7814 mRNA_7815 mRNA_7816
16180 16181 16182 16183 16184
auxotrophy Dropout Complete N.D. N.D. -0.6 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 -0.2 -0.1
growth on ypd YPD N.D. N.D. -0.2 -0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 -0.2 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 +0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 -0.3 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.1 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.1 -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.0 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.0 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 +0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.3 -0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.0 +0.2 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.0 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 -0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 +0.4 +0.0
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