Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16210 and 16211 are separated by 258 nucleotides16211 and 16212 are separated by 342 nucleotides16212 and 16213 are separated by 1555 nucleotides16213 and 16214 are separated by 266 nucleotides mRNA_7842: 16210 - HMMPfam-Ankyrin repeats (3 copies)-PF12796,ProSiteProfiles-Ankyrin repeat profile.-PS50088,ProSiteProfiles-Ankyrin repeat region circular profile.-PS50297,SMART-ankyrin repeats-SM00248,SUPERFAMILY--SSF48403, at 1,195,063 to 1,195,629 16210 mRNA_7843: 16211 - BLAST alpha- -glucan [Moniliophthora roreri MCA 2997], at 1,195,888 to 1,196,592 16211 mRNA_7844: 16212 - K02966 RP-S19e, RPS19 small subunit ribosomal protein S19e, at 1,196,935 to 1,198,284 16212 mRNA_7845: 16213 - K14154 THI6 thiamine-phosphate diphosphorylase / hydroxyethylthiazole kinase, at 1,199,840 to 1,202,125 16213 mRNA_7846: 16214 - K04460 PPP5C serine/threonine-protein phosphatase 5, at 1,202,392 to 1,204,902 16214
Group Condition mRNA_7842 mRNA_7843 mRNA_7844 mRNA_7845 mRNA_7846
16210 16211 16212 16213 16214
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. -0.2 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. -0.1 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. -0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. N.D. -0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. -0.0 -0.0
growth on ypd YPD -0.3 N.D. N.D. +0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.2 -0.1
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. +0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. -0.1 +0.2
auxotrophy Dropout Complete +0.5 N.D. N.D. +0.2 -0.2
auxotrophy YNB Glucose +0.5 N.D. N.D. +0.1 +0.0
growth on ricinoleic acid YNB Glucose +0.9 N.D. N.D. +0.0 +0.1
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