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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
16213 and 16214 are separated by 266 nucleotides
16214 and 16215 are separated by 1082 nucleotides
16215 and 16216 are separated by 606 nucleotides
16216 and 16217 are separated by 4639 nucleotides
mRNA_7845: 16213 - K14154 THI6 thiamine-phosphate diphosphorylase / hydroxyethylthiazole kinase, at 1,199,840 to 1,202,125
16213
mRNA_7846: 16214 - K04460 PPP5C serine/threonine-protein phosphatase 5, at 1,202,392 to 1,204,902
16214
mRNA_7847: 16215 - KOG0085 G protein subunit Galphaq/Galphay, small G protein superfamily, at 1,205,985 to 1,207,828
16215
mRNA_7848: 16216 - BLAST LYR MOTIF-CONTAINING PROTEIN 7 [Ceraceosorus bombacis], at 1,208,435 to 1,209,073
16216
mRNA_7849: 16217 - KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate sulfurtransferase, at 1,213,713 to 1,217,281
16217
Group
Condition
mRNA
_7845
mRNA
_7846
mRNA
_7847
mRNA
_7848
mRNA
_7849
16213
16214
16215
16216
16217
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.1
-1.0
N.D.
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.1
-0.4
-0.3
N.D.
-0.2
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.0
-0.3
N.D.
-0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.3
-0.5
+0.2
N.D.
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
-0.2
-0.3
N.D.
-0.1
growth on oleic acid
YNB Oleic Acid
-0.0
-0.1
-0.2
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
-0.5
+0.6
N.D.
-0.2
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
-0.4
-0.0
N.D.
+0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.1
-0.2
+0.2
N.D.
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
-0.3
+0.6
N.D.
-0.0
auxotrophy
YNB Glucose 75mM Methionine
+0.1
-0.1
+0.3
N.D.
-0.4
ypd buoyancy
40 hrs High Buoyancy
-0.1
+0.0
+0.0
N.D.
-0.0
ypd buoyancy
40 hrs Median Buoyancy
-0.0
-0.0
+0.1
N.D.
-0.1
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
+0.2
+0.4
N.D.
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.0
+0.0
+0.3
N.D.
-0.2
auxotrophy
YNB Glucose
+0.1
+0.0
+0.3
N.D.
-0.1
growth on oleic acid
YNB Glucose
-0.0
-0.0
+0.6
N.D.
-0.1
growth on ricinoleic acid
YNB Glucose
+0.0
+0.1
+0.5
N.D.
-0.1
auxotrophy
Dropout Complete
+0.2
-0.2
+0.5
N.D.
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
-0.1
+0.7
N.D.
-0.1
growth on ypd
YPD
+0.1
+0.2
+0.7
N.D.
-0.1
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