Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16213 and 16214 are separated by 266 nucleotides16214 and 16215 are separated by 1082 nucleotides16215 and 16216 are separated by 606 nucleotides16216 and 16217 are separated by 4639 nucleotides mRNA_7845: 16213 - K14154 THI6 thiamine-phosphate diphosphorylase / hydroxyethylthiazole kinase, at 1,199,840 to 1,202,125 16213 mRNA_7846: 16214 - K04460 PPP5C serine/threonine-protein phosphatase 5, at 1,202,392 to 1,204,902 16214 mRNA_7847: 16215 - KOG0085 G protein subunit Galphaq/Galphay, small G protein superfamily, at 1,205,985 to 1,207,828 16215 mRNA_7848: 16216 - BLAST LYR MOTIF-CONTAINING PROTEIN 7 [Ceraceosorus bombacis], at 1,208,435 to 1,209,073 16216 mRNA_7849: 16217 - KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate sulfurtransferase, at 1,213,713 to 1,217,281 16217
Group Condition mRNA_7845 mRNA_7846 mRNA_7847 mRNA_7848 mRNA_7849
16213 16214 16215 16216 16217
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.1 -1.0 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.4 -0.3 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 -0.3 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.5 +0.2 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.2 -0.3 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.0 -0.1 -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.5 +0.6 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.4 -0.0 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.2 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.3 +0.6 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 +0.3 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 +0.0 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 +0.1 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.2 +0.4 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.0 +0.3 N.D. -0.2
auxotrophy YNB Glucose +0.1 +0.0 +0.3 N.D. -0.1
growth on oleic acid YNB Glucose -0.0 -0.0 +0.6 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.0 +0.1 +0.5 N.D. -0.1
auxotrophy Dropout Complete +0.2 -0.2 +0.5 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 +0.7 N.D. -0.1
growth on ypd YPD +0.1 +0.2 +0.7 N.D. -0.1
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