Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16221 and 16222 are separated by 486 nucleotides16222 and 16223 are separated by 397 nucleotides16223 and 16224 are separated by 94 nucleotides16224 and 16225 are separated by 699 nucleotides mRNA_7853: 16221 - Hypothetical Protein, at 1,226,936 to 1,227,400 16221 mRNA_7854: 16222 - HMMPfam-Fungal protein of unknown function (DUF2015)-PF09435, at 1,227,887 to 1,228,457 16222 mRNA_7855: 16223 - K15443 TRM82, WDR4 tRNA (guanine-N(7)-)-methyltransferase subunit TRM82, at 1,228,855 to 1,230,795 16223 mRNA_7856: 16224 - K17783 ERV1, GFER, ALR mitochondrial FAD-linked sulfhydryl oxidase, at 1,230,890 to 1,231,576 16224 mRNA_7857: 16225 - K00211 TYR1 prephenate dehydrogenase (NADP+), at 1,232,276 to 1,233,897 16225
Group Condition mRNA_7853 mRNA_7854 mRNA_7855 mRNA_7856 mRNA_7857
16221 16222 16223 16224 16225
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -1.7 -0.7 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -1.9 -0.5 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.4 -1.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.6 -1.1 N.D. N.D.
growth on ypd YPD -0.0 -0.1 -1.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.3 -1.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.7 -1.0 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.8 -0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.1 +0.4 -1.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.2 -1.8 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.9 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.2 -0.6 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.2 -0.0 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 -0.4 +0.1 N.D. N.D.
auxotrophy Dropout Complete -0.1 +0.1 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.3 -0.3 N.D. N.D.
auxotrophy YNB Glucose -0.1 -0.2 +0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.5 +0.9 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 +0.6 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.2 +0.2 +0.8 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +1.4 N.D. N.D.
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