Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16223 and 16224 are separated by 94 nucleotides16224 and 16225 are separated by 699 nucleotides16225 and 16226 are separated by 2090 nucleotides16226 and 16227 are separated by 202 nucleotides mRNA_7855: 16223 - K15443 TRM82, WDR4 tRNA (guanine-N(7)-)-methyltransferase subunit TRM82, at 1,228,855 to 1,230,795 16223 mRNA_7856: 16224 - K17783 ERV1, GFER, ALR mitochondrial FAD-linked sulfhydryl oxidase, at 1,230,890 to 1,231,576 16224 mRNA_7857: 16225 - K00211 TYR1 prephenate dehydrogenase (NADP+), at 1,232,276 to 1,233,897 16225 mRNA_7858: 16226 - K11673 ACTR8, ARP8, INO80N actin-related protein 8, at 1,235,988 to 1,238,958 16226 mRNA_7859: 16227 - Hypothetical Protein, at 1,239,161 to 1,241,466 16227
Group Condition mRNA_7855 mRNA_7856 mRNA_7857 mRNA_7858 mRNA_7859
16223 16224 16225 16226 16227
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. N.D. -3.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.7 N.D. N.D. -0.6 -0.1
auxotrophy YNB Glucose 75mM Arginine -1.1 N.D. N.D. -0.5 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.0 N.D. N.D. -0.8 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 N.D. N.D. -0.4 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.8 N.D. N.D. +0.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. -0.8 -0.4
auxotrophy Dropout Complete -0.2 N.D. N.D. -0.2 -0.4
growth on oleic acid YNB Oleic Acid -1.5 N.D. N.D. +0.8 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. N.D. +0.3 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 N.D. N.D. -0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. +0.0 -0.3
auxotrophy YNB Glucose +0.3 N.D. N.D. -0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. -0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. +0.7 -0.0
ypd buoyancy 40 hrs Median Buoyancy +1.4 N.D. N.D. -0.9 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.9 N.D. N.D. -0.5 +0.1
growth on ypd YPD -1.5 N.D. N.D. +2.0 +0.2
growth on ricinoleic acid YNB Glucose +0.8 N.D. N.D. +0.1 -0.2
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +1.2 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 N.D. N.D. +1.0 -0.1
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