Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16236 and 16237 are separated by 188 nucleotides16237 and 16238 are separated by 384 nucleotides16238 and 16239 are separated by 504 nucleotides16239 and 16240 are separated by 275 nucleotides mRNA_7868: 16236 - Hypothetical Protein, at 1,262,755 to 1,264,857 16236 mRNA_7869: 16237 - KOG4366 Predicted thioesterase, at 1,265,046 to 1,266,017 16237 mRNA_7870: 16238 - HMMPfam-Putative S-adenosyl-L-methionine-dependent methyltransferase-PF02636,SUPERFAMILY--SSF53335, at 1,266,402 to 1,268,243 16238 mRNA_7871: 16239 - HMMPfam-NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-PF10785,HMMPfam-C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit-PF12853, at 1,268,748 to 1,269,594 16239 mRNA_7872: 16240 - K11411 SIRT1, SIR2L1 NAD-dependent deacetylase sirtuin 1, at 1,269,870 to 1,272,263 16240
Group Condition mRNA_7868 mRNA_7869 mRNA_7870 mRNA_7871 mRNA_7872
16236 16237 16238 16239 16240
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -2.6 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -1.7 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -1.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.4 -1.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 -1.3 N.D. N.D. +0.1
growth on ypd YPD -0.6 -0.1 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -1.1 -0.3 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.2 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 -0.2 N.D. N.D. +0.3
growth on ricinoleic acid YNB Glucose -0.2 -0.1 N.D. N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.1 N.D. N.D. +0.3
growth on oleic acid YNB Glucose -0.1 +0.2 N.D. N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.5 N.D. N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.5 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.5 +1.1 N.D. N.D. +0.3
auxotrophy Dropout Complete -0.1 +0.9 N.D. N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +1.1 N.D. N.D. +0.1
auxotrophy YNB Glucose -0.1 +1.0 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +1.2 N.D. N.D. +0.3
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