Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16238 and 16239 are separated by 504 nucleotides16239 and 16240 are separated by 275 nucleotides16240 and 16241 are separated by 163 nucleotides16241 and 16242 are separated by 147 nucleotides mRNA_7870: 16238 - HMMPfam-Putative S-adenosyl-L-methionine-dependent methyltransferase-PF02636,SUPERFAMILY--SSF53335, at 1,266,402 to 1,268,243 16238 mRNA_7871: 16239 - HMMPfam-NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-PF10785,HMMPfam-C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit-PF12853, at 1,268,748 to 1,269,594 16239 mRNA_7872: 16240 - K11411 SIRT1, SIR2L1 NAD-dependent deacetylase sirtuin 1, at 1,269,870 to 1,272,263 16240 mRNA_7873: 16241 - K10258 TER, TSC13, CER10 very-long-chain enoyl-CoA reductase, at 1,272,427 to 1,274,098 16241 mRNA_7874: 16242 - K02202 CDK7 cyclin-dependent kinase 7, at 1,274,246 to 1,275,705 16242
Group Condition mRNA_7870 mRNA_7871 mRNA_7872 mRNA_7873 mRNA_7874
16238 16239 16240 16241 16242
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.4 N.D. -1.3
growth on ypd YPD N.D. N.D. -0.1 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. -0.2
auxotrophy YNB Glucose N.D. N.D. +0.2 N.D. -0.4
auxotrophy Dropout Complete N.D. N.D. +0.3 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.2 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.0 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.6 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 N.D. +0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.4 N.D. +0.9
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