Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16239 and 16240 are separated by 275 nucleotides16240 and 16241 are separated by 163 nucleotides16241 and 16242 are separated by 147 nucleotides16242 and 16243 are separated by 438 nucleotides mRNA_7871: 16239 - HMMPfam-NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit-PF10785,HMMPfam-C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit-PF12853, at 1,268,748 to 1,269,594 16239 mRNA_7872: 16240 - K11411 SIRT1, SIR2L1 NAD-dependent deacetylase sirtuin 1, at 1,269,870 to 1,272,263 16240 mRNA_7873: 16241 - K10258 TER, TSC13, CER10 very-long-chain enoyl-CoA reductase, at 1,272,427 to 1,274,098 16241 mRNA_7874: 16242 - K02202 CDK7 cyclin-dependent kinase 7, at 1,274,246 to 1,275,705 16242 mRNA_7875: 16243 - HMMPfam-Protein of unknown function (DUF4246)-PF14033, at 1,276,144 to 1,278,283 16243
Group Condition mRNA_7871 mRNA_7872 mRNA_7873 mRNA_7874 mRNA_7875
16239 16240 16241 16242 16243
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -1.3 +0.1
growth on ypd YPD N.D. -0.1 N.D. -0.6 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -0.6 -0.0
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.4 -0.2
auxotrophy Dropout Complete N.D. +0.3 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. -0.2 +0.0
growth on oleic acid YNB Glucose N.D. +0.2 N.D. -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 N.D. -0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. -0.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. +0.6 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.5 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. +0.9 +0.1
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