Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16243 and 16244 are separated by 123 nucleotides16244 and 16245 are separated by 308 nucleotides16245 and 16246 are separated by 221 nucleotides16246 and 16247 are separated by 5 nucleotides mRNA_7875: 16243 - HMMPfam-Protein of unknown function (DUF4246)-PF14033, at 1,276,144 to 1,278,283 16243 mRNA_7876: 16244 - HMMPfam-Protein of unknown function (DUF4246)-PF14033, at 1,278,407 to 1,280,463 16244 mRNA_7877: 16245 - KOG1575 Voltage-gated shaker-like K+ channel, subunit beta/KCNAB, at 1,280,772 to 1,282,226 16245 mRNA_7878: 16246 - K14400 PCF11 pre-mRNA cleavage complex 2 protein Pcf11, at 1,282,448 to 1,285,814 16246 mRNA_7879: 16247 - Hypothetical Protein, at 1,285,820 to 1,286,086 16247
Group Condition mRNA_7875 mRNA_7876 mRNA_7877 mRNA_7878 mRNA_7879
16243 16244 16245 16246 16247
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -1.4 -1.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.2 -0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.1 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 -0.2 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.2 +0.3 -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 +0.2 -0.1 N.D. N.D.
growth on ypd YPD -0.2 -0.3 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 +0.1 -0.1 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 -0.1 N.D. N.D.
auxotrophy YNB Glucose -0.2 +0.6 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.3 +0.1 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.6 -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.2 +0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 +0.3 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.5 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.5 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.6 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 +0.2 N.D. N.D.
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