Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16265 and 16266 are separated by 218 nucleotides16266 and 16267 are separated by 269 nucleotides16267 and 16268 are separated by 486 nucleotides16268 and 16269 are separated by 1271 nucleotides mRNA_7897: 16265 - K10872 DMC1 meiotic recombination protein DMC1, at 1,325,676 to 1,327,786 16265 mRNA_7898: 16266 - K12660 rhmA 2-dehydro-3-deoxy-L-rhamnonate aldolase, at 1,328,005 to 1,329,095 16266 mRNA_7899: 16267 - K00618 E2.3.1.1 amino-acid N-acetyltransferase, at 1,329,365 to 1,331,583 16267 mRNA_7900: 16268 - Hypothetical Protein, at 1,332,070 to 1,333,846 16268 mRNA_7901: 16269 - KOG1605 TFIIF-interacting CTD phosphatase, including NLI-interacting factor (involved in RNA polymerase II regulation), at 1,335,118 to 1,336,063 16269
Group Condition mRNA_7897 mRNA_7898 mRNA_7899 mRNA_7900 mRNA_7901
16265 16266 16267 16268 16269
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.0 -1.8 -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.9 -1.7 +0.1 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.1 -2.1 +0.9 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -1.7 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.7 -1.5 +0.7 +0.7
growth on oleic acid YNB Oleic Acid +0.3 -0.3 -1.8 +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.4 -1.9 +0.5 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.2 +0.3 -0.4 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.6 -1.6 +0.5 +0.9
growth on ricinoleic acid YNB Glucose -0.1 -0.5 -1.1 -0.1 +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.5 -1.1 +0.1 +0.9
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 -0.3 +0.2 -0.2
growth on oleic acid YNB Glucose +0.2 -0.0 -2.1 +0.2 +1.3
growth on ypd YPD -0.1 -0.5 -0.1 +0.5 -0.0
auxotrophy YNB Glucose +0.0 +0.1 -2.1 +1.3 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 -0.5 -0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.5 -2.1 +0.3 +1.2
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.2 -0.1 -0.0 +0.4
auxotrophy Dropout Complete +0.1 -0.6 -0.3 +0.8 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.0 -0.3 +1.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.1 -0.5 +0.4 +1.1
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