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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
16265 and 16266 are separated by 218 nucleotides
16266 and 16267 are separated by 269 nucleotides
16267 and 16268 are separated by 486 nucleotides
16268 and 16269 are separated by 1271 nucleotides
mRNA_7897: 16265 - K10872 DMC1 meiotic recombination protein DMC1, at 1,325,676 to 1,327,786
16265
mRNA_7898: 16266 - K12660 rhmA 2-dehydro-3-deoxy-L-rhamnonate aldolase, at 1,328,005 to 1,329,095
16266
mRNA_7899: 16267 - K00618 E2.3.1.1 amino-acid N-acetyltransferase, at 1,329,365 to 1,331,583
16267
mRNA_7900: 16268 - Hypothetical Protein, at 1,332,070 to 1,333,846
16268
mRNA_7901: 16269 - KOG1605 TFIIF-interacting CTD phosphatase, including NLI-interacting factor (involved in RNA polymerase II regulation), at 1,335,118 to 1,336,063
16269
Group
Condition
mRNA
_7897
mRNA
_7898
mRNA
_7899
mRNA
_7900
mRNA
_7901
16265
16266
16267
16268
16269
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
+0.0
-1.8
-0.0
-0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.9
-1.7
+0.1
+0.6
auxotrophy
YNB Glucose 75mM Methionine
+0.3
-0.1
-2.1
+0.9
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
+0.1
-1.7
+0.3
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
-0.7
-1.5
+0.7
+0.7
growth on oleic acid
YNB Oleic Acid
+0.3
-0.3
-1.8
+0.4
+0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
-0.4
-1.9
+0.5
+0.8
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
+0.2
+0.3
-0.4
-0.6
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.1
-0.6
-1.6
+0.5
+0.9
growth on ricinoleic acid
YNB Glucose
-0.1
-0.5
-1.1
-0.1
+1.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
-0.5
-1.1
+0.1
+0.9
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.1
-0.3
+0.2
-0.2
growth on oleic acid
YNB Glucose
+0.2
-0.0
-2.1
+0.2
+1.3
growth on ypd
YPD
-0.1
-0.5
-0.1
+0.5
-0.0
auxotrophy
YNB Glucose
+0.0
+0.1
-2.1
+1.3
+0.3
ypd buoyancy
40 hrs Median Buoyancy
+0.1
-0.0
-0.5
-0.0
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
+0.5
-2.1
+0.3
+1.2
ypd buoyancy
40 hrs High Buoyancy
+0.1
-0.2
-0.1
-0.0
+0.4
auxotrophy
Dropout Complete
+0.1
-0.6
-0.3
+0.8
+0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.1
-0.0
-0.3
+1.3
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.2
-0.1
-0.5
+0.4
+1.1
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