Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16266 and 16267 are separated by 269 nucleotides16267 and 16268 are separated by 486 nucleotides16268 and 16269 are separated by 1271 nucleotides16269 and 16270 are separated by 2352 nucleotides mRNA_7898: 16266 - K12660 rhmA 2-dehydro-3-deoxy-L-rhamnonate aldolase, at 1,328,005 to 1,329,095 16266 mRNA_7899: 16267 - K00618 E2.3.1.1 amino-acid N-acetyltransferase, at 1,329,365 to 1,331,583 16267 mRNA_7900: 16268 - Hypothetical Protein, at 1,332,070 to 1,333,846 16268 mRNA_7901: 16269 - KOG1605 TFIIF-interacting CTD phosphatase, including NLI-interacting factor (involved in RNA polymerase II regulation), at 1,335,118 to 1,336,063 16269 mRNA_7902: 16270 - K13348 MPV17 protein Mpv17, at 1,338,416 to 1,339,607 16270
Group Condition mRNA_7898 mRNA_7899 mRNA_7900 mRNA_7901 mRNA_7902
16266 16267 16268 16269 16270
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -1.7 +0.1 +0.6 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -1.8 -0.0 -0.2 +0.6
growth on oleic acid YNB Oleic Acid -0.3 -1.8 +0.4 +0.4 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -2.1 +0.9 -0.3 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -1.7 +0.3 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 -1.1 +0.1 +0.9 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -1.9 +0.5 +0.8 +0.0
growth on ricinoleic acid YNB Glucose -0.5 -1.1 -0.1 +1.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 -1.5 +0.7 +0.7 +0.1
growth on ypd YPD -0.5 -0.1 +0.5 -0.0 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.3 +0.2 -0.2 -0.3
growth on oleic acid YNB Glucose -0.0 -2.1 +0.2 +1.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -1.6 +0.5 +0.9 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.3 -0.4 -0.6 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.5 -0.0 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 -0.0 +0.4 +0.0
auxotrophy YNB Glucose +0.1 -2.1 +1.3 +0.3 +0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -2.1 +0.3 +1.2 +0.4
auxotrophy Dropout Complete -0.6 -0.3 +0.8 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.5 +0.4 +1.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.3 +1.3 -0.2 +0.3
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