Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16267 and 16268 are separated by 486 nucleotides16268 and 16269 are separated by 1271 nucleotides16269 and 16270 are separated by 2352 nucleotides16270 and 16271 are separated by 211 nucleotides mRNA_7899: 16267 - K00618 E2.3.1.1 amino-acid N-acetyltransferase, at 1,329,365 to 1,331,583 16267 mRNA_7900: 16268 - Hypothetical Protein, at 1,332,070 to 1,333,846 16268 mRNA_7901: 16269 - KOG1605 TFIIF-interacting CTD phosphatase, including NLI-interacting factor (involved in RNA polymerase II regulation), at 1,335,118 to 1,336,063 16269 mRNA_7902: 16270 - K13348 MPV17 protein Mpv17, at 1,338,416 to 1,339,607 16270 mRNA_7903: 16271 - K14810 DDX56, DBP9 ATP-dependent RNA helicase DDX56/DBP9, at 1,339,819 to 1,342,318 16271
Group Condition mRNA_7899 mRNA_7900 mRNA_7901 mRNA_7902 mRNA_7903
16267 16268 16269 16270 16271
facs on lipid accumulation Sorted Low BODIPY Gate -1.8 -0.0 -0.2 +0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.7 +0.3 -0.0 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.7 +0.1 +0.6 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -2.1 +0.9 -0.3 +0.4 N.D.
growth on oleic acid YNB Oleic Acid -1.8 +0.4 +0.4 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.4 -0.6 -0.1 N.D.
growth on oleic acid YNB Glucose -2.1 +0.2 +1.3 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.2 -0.2 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.9 +0.5 +0.8 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.1 +0.1 +0.9 -0.4 N.D.
growth on ricinoleic acid YNB Glucose -1.1 -0.1 +1.2 -0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 -0.0 +0.2 -0.0 N.D.
growth on ypd YPD -0.1 +0.5 -0.0 -0.5 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -2.1 +0.3 +1.2 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.5 +0.7 +0.7 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.6 +0.5 +0.9 +0.1 N.D.
auxotrophy YNB Glucose -2.1 +1.3 +0.3 +0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 +0.4 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.4 +1.1 -0.2 N.D.
auxotrophy Dropout Complete -0.3 +0.8 +0.2 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.3 +1.3 -0.2 +0.3 N.D.
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