Fitness for 4 genes in Rhodosporidium toruloides IFO0880

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500 nt 16279 and 16280 are separated by 760 nucleotides16280 and 16281 are separated by 176 nucleotides16281 and 16282 are separated by 1224 nucleotides mRNA_7911: 16279 - K03015 RPB7, POLR2G DNA-directed RNA polymerase II subunit RPB7, at 1,441 to 2,386 16279 mRNA_7912: 16280 - K11434 PRMT1 type I protein arginine methyltransferase, at 3,147 to 4,682 16280 mRNA_7913: 16281 - K00234 SDHA, SDH1 succinate dehydrogenase (ubiquinone) flavoprotein subunit, at 4,859 to 7,568 16281 mRNA_7914: 16282 - K10684 UBLE1A, SAE1 ubiquitin-like 1-activating enzyme E1 A, at 8,793 to 10,247 16282
Group Condition mRNA_7911 mRNA_7912 mRNA_7913 mRNA_7914
16279 16280 16281 16282
facs on lipid accumulation Sorted High BODIPY Gate N.D. -3.6 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -2.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -2.4 N.D. N.D.
auxotrophy YNB Glucose N.D. -1.8 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -1.7 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.6 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.3 N.D. N.D.
auxotrophy Dropout Complete N.D. -1.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.7 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.6 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 N.D. N.D.
growth on ypd YPD N.D. +0.9 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +1.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +1.4 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +2.3 N.D. N.D.
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