Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16279 and 16280 are separated by 760 nucleotides16280 and 16281 are separated by 176 nucleotides16281 and 16282 are separated by 1224 nucleotides16282 and 16283 are separated by 1881 nucleotides mRNA_7911: 16279 - K03015 RPB7, POLR2G DNA-directed RNA polymerase II subunit RPB7, at 1,441 to 2,386 16279 mRNA_7912: 16280 - K11434 PRMT1 type I protein arginine methyltransferase, at 3,147 to 4,682 16280 mRNA_7913: 16281 - K00234 SDHA, SDH1 succinate dehydrogenase (ubiquinone) flavoprotein subunit, at 4,859 to 7,568 16281 mRNA_7914: 16282 - K10684 UBLE1A, SAE1 ubiquitin-like 1-activating enzyme E1 A, at 8,793 to 10,247 16282 mRNA_7915: 16283 - SUPERFAMILY--SSF51445, at 12,129 to 14,300 16283
Group Condition mRNA_7911 mRNA_7912 mRNA_7913 mRNA_7914 mRNA_7915
16279 16280 16281 16282 16283
facs on lipid accumulation Sorted High BODIPY Gate N.D. -3.6 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -2.4 N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -2.5 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -1.7 N.D. N.D. -0.4
auxotrophy YNB Glucose N.D. -1.8 N.D. N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.6 N.D. N.D. +0.0
auxotrophy Dropout Complete N.D. -1.3 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.6 N.D. N.D. -0.5
growth on oleic acid YNB Glucose N.D. -0.7 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.3 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 N.D. N.D. -0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 N.D. N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.7 N.D. N.D. -0.0
growth on ypd YPD N.D. +0.9 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +1.2 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +1.4 N.D. N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +2.3 N.D. N.D. +0.4
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