Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16280 and 16281 are separated by 176 nucleotides16281 and 16282 are separated by 1224 nucleotides16282 and 16283 are separated by 1881 nucleotides16283 and 16284 are separated by 383 nucleotides mRNA_7912: 16280 - K11434 PRMT1 type I protein arginine methyltransferase, at 3,147 to 4,682 16280 mRNA_7913: 16281 - K00234 SDHA, SDH1 succinate dehydrogenase (ubiquinone) flavoprotein subunit, at 4,859 to 7,568 16281 mRNA_7914: 16282 - K10684 UBLE1A, SAE1 ubiquitin-like 1-activating enzyme E1 A, at 8,793 to 10,247 16282 mRNA_7915: 16283 - SUPERFAMILY--SSF51445, at 12,129 to 14,300 16283 mRNA_7916: 16284 - K08683 HSD17B10 3-hydroxyacyl-CoA dehydrogenase / 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 14,684 to 15,975 16284
Group Condition mRNA_7912 mRNA_7913 mRNA_7914 mRNA_7915 mRNA_7916
16280 16281 16282 16283 16284
facs on lipid accumulation Sorted High BODIPY Gate -3.6 N.D. N.D. +0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -2.5 N.D. N.D. +0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine -2.4 N.D. N.D. -0.2 -0.4
auxotrophy YNB Glucose -1.8 N.D. N.D. -0.2 -0.8
auxotrophy YNB Glucose 75mM Arginine -1.7 N.D. N.D. -0.4 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -1.6 N.D. N.D. +0.0 -0.1
auxotrophy Dropout Complete -1.3 N.D. N.D. +0.1 -0.3
growth on oleic acid YNB Glucose -0.7 N.D. N.D. -0.1 -0.2
growth on oleic acid YNB Oleic Acid -0.6 N.D. N.D. -0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 N.D. N.D. +0.5 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. -0.4 -0.4
growth on ricinoleic acid YNB Glucose +0.0 N.D. N.D. +0.2 -0.3
growth on ypd YPD +0.9 N.D. N.D. +0.1 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. N.D. -0.9 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. +0.0 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. N.D. -0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy +1.4 N.D. N.D. -0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy +1.2 N.D. N.D. +0.1 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +2.3 N.D. N.D. +0.4 +0.8
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