Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16282 and 16283 are separated by 1881 nucleotides16283 and 16284 are separated by 383 nucleotides16284 and 16285 are separated by 374 nucleotides16285 and 16286 are separated by 194 nucleotides mRNA_7914: 16282 - K10684 UBLE1A, SAE1 ubiquitin-like 1-activating enzyme E1 A, at 8,793 to 10,247 16282 mRNA_7915: 16283 - SUPERFAMILY--SSF51445, at 12,129 to 14,300 16283 mRNA_7916: 16284 - K08683 HSD17B10 3-hydroxyacyl-CoA dehydrogenase / 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 14,684 to 15,975 16284 mRNA_7917: 16285 - K18477 RMT2 type IV protein arginine methyltransferase, at 16,350 to 18,077 16285 mRNA_7918: 16286 - K15523 FN3KRP protein-ribulosamine 3-kinase, at 18,272 to 19,315 16286
Group Condition mRNA_7914 mRNA_7915 mRNA_7916 mRNA_7917 mRNA_7918
16282 16283 16284 16285 16286
auxotrophy YNB Glucose N.D. -0.2 -0.8 -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 +0.8 -2.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.8 -0.6 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 -0.6 +0.2 -0.3
growth on ypd YPD N.D. +0.1 -0.8 +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -0.2 -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.4 +0.1 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.3 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -1.0 +0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 -0.4 +0.3 +0.1
auxotrophy Dropout Complete N.D. +0.1 -0.3 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -0.1 -0.1 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.5 +0.1 -0.2 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.9 +0.6 +0.2 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.4 -0.3 +0.2
growth on oleic acid YNB Glucose N.D. -0.1 -0.2 +0.3 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.3 -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.6 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 +0.1 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.0 +0.3 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 +0.3 +0.2 +0.5
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