Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16284 and 16285 are separated by 374 nucleotides16285 and 16286 are separated by 194 nucleotides16286 and 16287 are separated by 3116 nucleotides16287 and 16288 are separated by 610 nucleotides mRNA_7916: 16284 - K08683 HSD17B10 3-hydroxyacyl-CoA dehydrogenase / 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 14,684 to 15,975 16284 mRNA_7917: 16285 - K18477 RMT2 type IV protein arginine methyltransferase, at 16,350 to 18,077 16285 mRNA_7918: 16286 - K15523 FN3KRP protein-ribulosamine 3-kinase, at 18,272 to 19,315 16286 mRNA_7919: 16287 - K01489 cdd, CDA cytidine deaminase, at 22,432 to 23,509 16287 mRNA_7920: 16288 - K02219 CKS1 cyclin-dependent kinase regulatory subunit CKS1, at 24,120 to 24,922 16288
Group Condition mRNA_7916 mRNA_7917 mRNA_7918 mRNA_7919 mRNA_7920
16284 16285 16286 16287 16288
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 -0.2 -1.5 +0.1
auxotrophy YNB Glucose -0.8 -0.3 -0.3 -0.7 +0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.3 -0.1 -0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.1 -0.2 -0.6 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -2.4 -0.1 +0.4 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.6 +0.2 -0.3 -0.4 +0.3
growth on oleic acid YNB Glucose -0.2 +0.3 +0.4 -0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 +0.3 -0.3 -0.0
growth on ypd YPD -0.8 +0.1 -0.1 +0.1 +0.7
growth on oleic acid YNB Oleic Acid +0.1 -0.2 +0.5 -0.4 +0.0
auxotrophy Dropout Complete -0.3 -0.1 +0.1 -0.0 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 -0.6 +0.0 +0.8 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 +0.2 +0.5 +0.2 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.1 +0.0 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.3 +0.1 +0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.3 +0.2 +0.0 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.2 +0.5 -0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.0 -0.1 +0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.6 -0.2 +0.1 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.3 +0.6 +0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.2 +0.3 +0.5 -0.1
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