Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16318 and 16319 are separated by 550 nucleotides16319 and 16320 are separated by 251 nucleotides16320 and 16321 are separated by 1033 nucleotides16321 and 16322 are separated by 544 nucleotides mRNA_7950: 16318 - K04802 PCNA proliferating cell nuclear antigen, at 76,989 to 79,257 16318 mRNA_7951: 16319 - K09527 DNAJC7 DnaJ homolog subfamily C member 7, at 79,808 to 82,314 16319 mRNA_7952: 16320 - KOG0627 Heat shock transcription factor, at 82,566 to 84,687 16320 mRNA_7953: 16321 - K13667 RUMI, KTELC1 protein glucosyltransferase, at 85,721 to 88,276 16321 mRNA_7954: 16322 - K01282 E3.4.14.- dipeptidyl aminopeptidase, at 88,821 to 92,965 16322
Group Condition mRNA_7950 mRNA_7951 mRNA_7952 mRNA_7953 mRNA_7954
16318 16319 16320 16321 16322
growth on ricinoleic acid YNB Glucose -1.0 -0.0 -0.0 -0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 +0.1 +0.1 +0.5 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.3 +0.0 +0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 -0.3 +0.2 -0.0
auxotrophy Dropout Complete -0.3 +0.0 -0.3 +0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 -0.3 +0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.9 +0.3 +0.1 +0.4 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +2.4 -0.9 -0.1 -0.3 -1.0
growth on oleic acid YNB Glucose -0.2 -0.1 +0.2 +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.1 +0.1 -0.1
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.0 +0.2 -0.0
auxotrophy YNB Glucose +0.4 +0.3 -0.6 +0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 -0.2 +0.5 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.3 -0.1 -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.0 -0.0 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.3 -0.1 +0.2 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.1 -0.2 +0.3 +0.3
growth on ypd YPD +0.1 +0.6 +0.1 +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.0 -0.1 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.6 -0.2 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.2 +0.3 -0.0 +0.2 -0.4
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