Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16320 and 16321 are separated by 1033 nucleotides16321 and 16322 are separated by 544 nucleotides16322 and 16323 are separated by 434 nucleotides16323 and 16324 are separated by 833 nucleotides mRNA_7952: 16320 - KOG0627 Heat shock transcription factor, at 82,566 to 84,687 16320 mRNA_7953: 16321 - K13667 RUMI, KTELC1 protein glucosyltransferase, at 85,721 to 88,276 16321 mRNA_7954: 16322 - K01282 E3.4.14.- dipeptidyl aminopeptidase, at 88,821 to 92,965 16322 mRNA_7955: 16323 - K00128 ALDH aldehyde dehydrogenase (NAD+), at 93,400 to 95,869 16323 mRNA_7956: 16324 - K03146 THI4, THI1 thiamine thiazole synthase, at 96,703 to 99,029 16324
Group Condition mRNA_7952 mRNA_7953 mRNA_7954 mRNA_7955 mRNA_7956
16320 16321 16322 16323 16324
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.3 -1.0 -0.7 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.2 -0.4 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 +0.0 -0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.0 -0.2 +0.0 -0.2
auxotrophy YNB Glucose -0.6 +0.2 +0.1 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.3 -0.2 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.2 -0.0 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 -0.0 -0.1 +0.1
auxotrophy Dropout Complete -0.3 +0.2 +0.0 -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 -0.1 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.3 +0.3 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 -0.2 -0.0 +0.2
growth on ypd YPD +0.1 +0.1 -0.0 -0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.0 -0.1 +0.3 +0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.5 +0.2 -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 -0.1 +0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 +0.4 +0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.0 +0.2 -0.0 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.5 +0.2 -0.2 +0.2
growth on oleic acid YNB Glucose +0.2 +0.2 +0.0 +0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.4 +0.2 +0.3 +0.1
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