Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16345 and 16346 are separated by 324 nucleotides16346 and 16347 are separated by 145 nucleotides16347 and 16348 are separated by 234 nucleotides16348 and 16349 are separated by 383 nucleotides mRNA_7977: 16345 - K17757 CARKD ATP-dependent NAD(P)H-hydrate dehydratase, at 134,234 to 135,920 16345 mRNA_7978: 16346 - K13341 PEX7, PTS2R peroxin-7, at 136,245 to 137,738 16346 mRNA_7979: 16347 - KOG0504 FOG Ankyrin repeat, at 137,884 to 138,763 16347 mRNA_7980: 16348 - KOG0550 Molecular chaperone (DnaJ superfamily), at 138,998 to 141,142 16348 mRNA_7981: 16349 - KOG2504 Monocarboxylate transporter, at 141,526 to 143,466 16349
Group Condition mRNA_7977 mRNA_7978 mRNA_7979 mRNA_7980 mRNA_7981
16345 16346 16347 16348 16349
growth on oleic acid YNB Oleic Acid -0.6 -4.2 N.D. -0.1 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 -3.3 N.D. +0.0 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -1.0 -2.3 N.D. -0.2 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -1.6 N.D. +0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.9 -0.6 N.D. +0.3 -0.1
growth on oleic acid YNB Glucose -1.0 +0.3 N.D. -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.7 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.5 N.D. -0.0 -0.4
auxotrophy YNB Glucose +0.4 -0.1 N.D. -0.3 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.6 N.D. -0.5 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.6 N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.3 N.D. -0.2 -0.2
growth on ricinoleic acid YNB Glucose -0.3 +0.5 N.D. -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 N.D. +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.3 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.1 N.D. +0.4 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.2 N.D. +0.0 +0.3
auxotrophy Dropout Complete +0.3 -0.0 N.D. +0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 N.D. +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.3 N.D. +0.1 +0.4
growth on ypd YPD -0.1 +0.8 N.D. +0.1 +0.1
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