Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16349 and 16350 are separated by 835 nucleotides16350 and 16351 are separated by 421 nucleotides16351 and 16352 are separated by 269 nucleotides16352 and 16353 are separated by 147 nucleotides mRNA_7981: 16349 - KOG2504 Monocarboxylate transporter, at 141,526 to 143,466 16349 mRNA_7982: 16350 - KOG2504 Monocarboxylate transporter, at 144,302 to 146,236 16350 mRNA_7983: 16351 - K11495 CENPA histone H3-like centromeric protein A, at 146,658 to 147,555 16351 mRNA_7984: 16352 - KOG3030 Lipid phosphate phosphatase and related enzymes of the PAP2 family, at 147,825 to 148,945 16352 mRNA_7985: 16353 - K15440 TAD1, ADAT1 tRNA-specific adenosine deaminase 1, at 149,093 to 150,954 16353
Group Condition mRNA_7981 mRNA_7982 mRNA_7983 mRNA_7984 mRNA_7985
16349 16350 16351 16352 16353
growth on oleic acid YNB Oleic Acid -0.6 -0.3 N.D. -0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.4 N.D. -0.1 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 +0.1 N.D. -0.0 +0.1
auxotrophy YNB Glucose -0.6 +0.1 N.D. -0.5 +0.2
growth on oleic acid YNB Glucose -0.2 -0.2 N.D. -0.5 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.0 N.D. -0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.1 N.D. -0.0 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 N.D. -0.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 +0.0 N.D. +0.0 +0.4
growth on ricinoleic acid YNB Glucose -0.2 +0.1 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 N.D. +0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.1 N.D. +0.3 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.0 N.D. +0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.1 N.D. +0.2 +0.1
auxotrophy Dropout Complete +0.0 +0.0 N.D. +0.0 +0.1
growth on ypd YPD +0.1 -0.2 N.D. -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.0 N.D. +0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.1 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.0 N.D. +0.0 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 +0.3 N.D. +0.5 -0.0
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